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Hair Loss News Archives
November 2003
Testosterone metabolism in human skin cells in
vitro and its interaction with estradiol and dutasteride.
Munster U, Hammer S, Blume-Peytavi U, Schafer-Korting M.
Abteilung fur Pharmakologie und Toxikologie, Institut fur Pharmazie,
Freie Universitat Berlin, Berlin, Deutschland.
Since the limited knowledge of cutaneous drug metabolism can impair
the development of specifically acting topical dermatics and
transdermal application systems, the cell-type-specific androgen
metabolism in human skin and its inhibition by drugs were
investigated.
Cultured human foreskin and scalp skin keratinocytes and fibroblasts as well as occipital scalp dermal papilla cells (DPC) were incubated with testosterone 10(-6) and 10(-8)M alone and in the presence of 17alpha-estradiol, 17beta-estradiol or dutasteride for 24 h.
Androgens extracted from culture supernatants were subjected to thin-layer chromatography and quantified by beta-counting. In keratinocytes and DPC, dihydrotestosterone (DHT) was only formed to a low extent while androstenedione was the main metabolite. In fibroblasts, DHT formation was pronounced following 10(-8)M testosterone.
Dutasteride 10(-8)M completely suppressed 5alpha-dihydro metabolite formation. 17alpha-Estradiol and 17beta-estradiol at nontoxic concentrations decreased 17-ketometabolites. Human skin regulates testosterone action by cell-type-specific activation or deactivation.
Effects of 17alpha-estradiol in androgenetic alopecia are not due to 5alpha-reductase inhibition.
Dutasteride may be useful in acne and androgenetic alopecia.
Copyright 2003 S. Karger AG, Basel